Liver Enzyme

Liver Enzyme Lab Purpose: The purpose of this experiment is to trounce a line the optimal temperature of Catalase. Procedure: Place a small core of blended coloured a baggie awake irrigate on a hotplate (or use scum if needed) to bring your water bath to your assigned temperature ( musical rhythm with thermometer) Place baggie with liver into water, brining both to coveted temperature. When ready, add 2 filter musical theater composition discs to the liver in the baggie and let it sit in the water for 3-4 minutes. Meanwhile, set up your apparatus, as follows, to measure O2 yield (volume in mL) *Perform dickens trials When ready, mix the peroxide with the discs, measure the volume of the splatter produced. Materials: -Thermometer -Filter paper discs -Inverted graduated cylinder -Hydrogen peroxide -Cafeteria tray -Rubber Stopper with tube -Gas detain bottle -Blender -Liver -Ziploc bag -Hotplate -Beakers Temperature (°C)T rial 1 (mL)Trial 2 (mL)Average (mL) 0120 140 one hundred thirty 10118115116.5 20118123120.5 30110115112.5 40171159165 501029297 60010050 7020010 80000 900.5126.

25 100000  Analysis: looking at the graph above, we notice overall the graph increases to a peak, and gone begins to decrease all the way downwards to zero. At 0 °C the shove along production is at roughly 130 mL, however at 10°C, 20°C and 30°C the gas production remains constant- ranging from 116.5mL to 120.5mL and then at long last to 112.5mL. At 40°C, there is a fulminant crucify in oxygen gas production, shootin g up to 165mL. after this boom, the amount o! f oxygen produced decreases at 50°C down to about 97mL. It continues to decrease at 60°C and 70°C going down to 50mL and then 10mL. lastly at 80°C, there is no oxygen gas produced further at 90°C we notice almost gas production, 6.25mL. The last temperature at 100°C decreases backIf you exigency to build a full essay, order it on our website: OrderCustomPaper.com

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